Subtilisin



Subtilisin is a serine protease. It is widely used commercially in detergents. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. The images at the left and at the right correspond to one representative Subtilisin, i.e. the crystal structure of native mature subtilisin from Thermococcus kodakarensis (2z2x).

Native mature subtilisin
2z2x – TkSub - Thermococcus kodakarensis

1sca, 1scb, 1sbc – BsSub - Bacillus subtilis (Subtilisin Carlsberg)

1gns, 1sup, 1st2, 1s01, 1sbt, 2sbt, 2st1 – BaSub - Bacillus amyloliquefaciens

1iav, 1gci, 1jea, 1st3 – BlSub - Bacillus lentus (Subtilisin Savinase)

1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus

2gko – Sub – psychrophilic Bacillus subtilis

Mutant mature subtilisin
3f49, 3co0, 2zrq – TkSubb (mutant)

1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant)

1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSub (mutant)

2xrm - BcSub residues 19-321 - Bacillus clausii

Unautoprocessed subtilisin (pro-Sub)
2z2z, 2e1p – pro-TkSub

2zwo, 2zwp – pro-TKSub (mutant)

2wv7 – pro-BcSub

2wwt, 2x8j - pro-BcSub (mutant)

Mutant unautoprocessed subtilisin
2zwo, 2zwp – pro-TKSub

Mature subtilisin complexed with its propeptide
3cnq – BaSub

1spb – BaSub (mutant)

3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub

1scj – BsSub

Subtilisin complexed with polypeptide inhibitor
1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4, 1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2

1tm5, 1tm7, 1tmg, 1to1, 1to2 - BaSub+ chymotrypsin inhibitor 2 (mutant)

3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI

1yu6 – BsSub+ovomucoid

1v5i – BaSubb+POIA1 peptide

1tk2, 1ndq – BlSub+gramicidin S

1r0r – BsSub+ovomucoid

1oyv – BsSub+tomato inhibitor II

1sbn, 1sib, 2sec, 1sni, 1sce – BsSub+Elgin C

2sni - BaSub+Elgin C2

1mee - BpSub+Elgin C – Bacillus pumilus

Subtilisin complexed with inhibitor
3bgo – BaSub+azide

1c3l – BsSub+Xe

1be8 – BsSub+cinnamoyl

1be6 - BsSub+cinnamoyl+acetonitrile

1bfk, 1scd – BsSub+acetonitrile

1bfu, 1af4 – BsSub+dioxane

1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives

1scn – BsSub+carbamate derivative

1bh6 – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone – Bacillus licheniformis

2wuv, 2wuw – BlSub fragment+acetonitrile

Selenosubtilisin
1ubn – BaSel-Sub

1sel – BsSel-Sub

Savinase
3bx1 – Sav – Bacillus lentus